Reporter

Part:BBa_J100263:Design

Designed by: Owen Koucky   Group: Campbell M Lab   (2016-06-09)


RS10 (Wachsmuth et al. 2013) in J119361


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 752
    Illegal AgeI site found at 864
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design of this sequence required the insertion of the RS10 riboswitch upstream of the reporter RFP gene. Such a design was made possible by the replacement of the GFP cassette in J119361 with the RS10 riboswitch via Golden Gate Assembly.


Source

The RS10 riboswitch was developed by Wachsumth et al. for their 2013 paper "De novo design of a synthetic riboswitch that regulates transcription termination." The RS10 riboswitch was inserted into J119361 via Golden Gate Assembly and replaced the GFP cassette that had occupied this part.

References